@article {15672,
	title = {In Situ Functionalization of Wet-Spun Fibre meshes for Bone Tissue Engineering},
	journal = {Journal of Tissue Engineering and Regenerative Medicine },
	volume = {5},
	year = {2011},
	month = {2011-02-01 00:00:00},
	pages = {104-111},
	publisher = {JOHN WILEY \& SONS LTD},
	abstract = {<p><span>Bone tissue engineering success strongly depends on our ability to develop new materials combining osteoconductive, osteoinductive and osteogenic properties. Recent studies suggest that biomaterials incorporating silanol (Si-OH) groups promote and maintain osteogenesis. The purpose of the present research work was to provide evidence that using wet-spinning technologies and a calcium silicate solution as a coagulation bath, it was possible to develop an in situ functionalization methodology to obtain 3D wet-spun fibre meshes with Si-OH groups, through a simple, economic and reliable process. SPCL (blend of starch with polycaprolactone) fibre meshes were produced by wet-spinning, using a calcium silicate solution as a non-solvent and functionalized in situ with Si-OH groups. In vitro tests, using goat bone marrow stromal cells (GBMSCs), showed that SPCL-Si scaffolds sustained cell viability and proliferation. Furthermore, high ALP activity and matrix production indicated that Si-OH groups improve cellular functionality towards the osteoblastic phenotype. Using this methodology, and assembling several wet-spun fibre meshes, 3D meshes can be developed, aiming at designing osteoconductive/osteoinductive 3D structures capable of stimulating bone ingrowth in vivo. Copyright (C) 2010 John Wiley \&amp; Sons, Ltd.</span></p>},
	keywords = {biodegradable scaffolds, Bone Tissue Engineering, marrow stromal cells, osteoconductive, silanol groups, wet-spinning},
	issn = {1932-6254},
	doi = {10.1002/term.294},
	url = {http://onlinelibrary.wiley.com/doi/10.1002/term.294/abstract},
	author = {Leonor, I. B. and Rodrigues, M. T. and Gomes, M. E. and Reis, R. L.}
}
