@article {15354, title = {Human Dermal Fibroblasts, Keratinocytes and Adipose Stem Cells Behaviour on Patterned Electrospun Nanofiber Meshes}, journal = {Tissue Engineering: Part A}, volume = {14,5}, year = {2010}, month = {2010-03-17 00:00:00}, pages = {742}, abstract = {

Natural extracellular Matrix (ECM) creates a unique cellular microenvironment. It acts as a support to organize cells in tissues, maintains their structure and works also as a reservoir for cytokines, thus controlling cell growth and differentiation. A well-defined biomaterial surface topography is believed to be adequate to mimic native ECM for guiding cell growth or tissue regeneration. This structure can be achieved by using an electrospinning technique, which allows producing a non-woven nanofibrous structure with topographic features mimicking the natural ECM.

This study evaluates the influence of micro-topography of pat- terned Polycaprolactone (PCL) nanofiber meshes, aimed at being used in skin regeneration approaches. The morphology, adhesion and proliferation of primary cultures of human keratinocytes (hKC), dermal fibroblasts (hDFs), and adipose-derived stem cells (hASCs), isolated from the abdominal region, was evaluated after seeding in those structures.

In vitro studies showed that the characteristic morphology of each cell type and respective phenotype was maintained on the patterned electrospun nanofiber meshes during the culture period. SEM micrographs demonstrated that these cells adhered better on the randomly distributed areas of the nanofibers than in the aligned ones. Furthermore, DNA quantification and metabolic activity analysis confirmed the enhanced performance of the cells adhered on the random structures. Additionally, the patterned areas were able to induce cell alignment along the nanofibers.

The combination of the organized and random structures into patterned nanofiber meshes, being able to control cell distribution and proliferation, showed promising characteristics for upcoming studies regarding skin tissue engineering applications.

}, keywords = {Adipose stem cells, Human Dermal Fibroblasts, Keratinocytes, nanofiber meshs}, author = {Cerqueira, M. T. and Martins, A. and Rada, T. and Gomes, M. E. and Marques, A. P. and Neves, N. M. and Reis, R. L.} }