@article {15206, title = {In Vitro Localization of Bone Growth Factors in Constructs of Biodegradable Scaffolds Seeded with Marrow Stromal Cells and Cultured in a Flow Perfusion Bioreactor}, journal = {Tissue Engineering}, volume = {12}, year = {2007}, month = {2007-05-21 00:00:00}, pages = {177}, abstract = {

Tissue engineering strategies aim at controlling the behavior of individual cells to stimulate tissue
formation. This control is achieved by mimicking signals that manage natural tissue development
or repair. Flow perfusion bioreactors that create culture environments with minimal diffusion constraints
and provide cells with mechanical stimulation may closely resemble in vivo conditions for
bone formation. Therefore, these culturing systems, in conjunction with an appropriate scaffold and
cell type, may provide significant insight towards the development of in vitro tissue engineering models
leading to improved strategies for the construction of bone tissue substitutes. The objective of
this study was to investigate the in vitro localization of several bone growth factors that are usually
associated with bone formation in vivo by culturing rat bone marrow stromal cells seeded onto
starch-based biodegradable fiber meshes in a flow perfusion bioreactor. The localization of several
bone-related growth factors{\textendash}namely, transforming growth factor-1, platelet-derived growth factor-
A, fibroblast growth factor-2, vascular endothelial growth factor, and bone morphogenetic protein-
2{\textendash}was determined at two different time points in scaffolds cultured under perfusion conditions
at two different flow rates using an immunohistochemistry technique. The results show the presence
of regions positively stained for all the growth factors considered, except platelet-derived growth
factor-A. Furthermore, the images obtained from the positively stained sections suggest an increase
in the immunohistochemically stained area at the higher flow rate and culture time. These observations
demonstrate that flow perfusion augments the functionality of scaffold/cell constructs grown
in vitro as it combines both biological and mechanical factors to enhance cell differentiation and cell
organization within the construct. This study also shows that flow perfusion bioreactor culture of
marrow stromal cells, combined with the use of appropriate biodegradable fiber meshes, may constitute
a useful model to study bone formation and assess bone tissue engineering strategies in vitro.

}, keywords = {Bone Growth Factors, TE}, author = {Gomes, M. E. and Bossano, C. M. and Johnston, C. M. and Reis, R. L. and Mikos, A. G.} }