@article {15201, title = {Effect of Flow Perfusion on the Osteogenic Differentiation of Bone Marrow Stromal Cells Cultured on Starch Based Three-Dimensional Scaffolds}, journal = {Journal of Biomedical Materials Research}, volume = {67A}, year = {2007}, month = {2007-05-21 00:00:00}, pages = {87}, abstract = {

This study aims to investigate the effect of culturing
conditions (static and flow perfusion) on the proliferation
and osteogenic differentiation of rat bone marrow
stromal cells seeded on two novel scaffolds exhibiting distinct
porous structures. Specifically, scaffolds based on
SEVA-C (a blend of starch with ethylene vinyl alcohol) and
SPCL (a blend of starch with polycaprolactone) were examined
in static and flow perfusion culture. SEVA-C scaffolds
were formed using an extrusion process, whereas SPCL
scaffolds were obtained by a fiber bonding process. For this
purpose, these scaffolds were seeded with marrow stromal
cells harvested from femoras and tibias of Wistar rats and
cultured in a flow perfusion bioreactor and in 6-well plates
for 3, 7, and 15 days. The proliferation and alkaline phosphatase
activity patterns were similar for both types of
scaffolds and for both culture conditions. However, calcium
content analysis revealed a significant enhancement of calcium
deposition on both scaffold types cultured under flow\ 

perfusion. This observation was confirmed by Von Kossastained
sections and tetracycline fluorescence. Histological
analysis and confocal images of the cultured scaffolds
showed a much better distribution of cells within the SPCL
scaffolds than the SEVA-C scaffolds, which had limited pore
interconnectivity, under flow perfusion conditions. In the
scaffolds cultured under static conditions, only a surface
layer of cells was observed. These results suggest that flow
perfusion culture enhances the osteogenic differentiation of
marrow stromal cells and improves their distribution in
three-dimensional, starch-based scaffolds. They also indicate
that scaffold architecture and especially pore interconnectivity
affect the homogeneity of the formed tissue.

}, keywords = {bone marrow stromal cells, Bone Tissue Engineering, flow perfusion bioreactor}, author = {Gomes, M. E. and Sikavitsas, V. I. and Behravesh, E. and Reis, R. L. and Mikos, A. G.} }